Cell Culture Facility
- Pamela Nakajima, PhD
- Joann Lutje, MS
Pamela Nakajima, PhD
- Pamela Nakajima, PhD
- Reimann R288
- Pricing &
- Contact Pamela Nakajima for pricing and scheduling.
The Cell Culture Facility has made it easier, more economical and more effective to use mammalian cell cultures in research at Fox Chase. Cell culture procedures provide the cellular materials for many of the molecular methods used to study normal and cancer cell proliferation, cellular regulatory mechanisms, and normal and abnormal development. As a result, cell culture has become a central technique in current molecular and cell biological research.
The Cell Culture Facility provides a wide range of services and technical assistance that are tailored to the individual needs of the user. The facility provides basic support services (preparation of numerous culture media and a source of culture supplies and sterile reagents), cell propagation services, expertise in establishing primary cultures from human and animal tissue samples, cell transformation services, screening services for detecting mycoplasma contamination in cultured cells, consultation to assist in experimental design, and sterile workstations for use by investigators who do not have facilities of their own. The cell culture facility is used by more than three-quarters of all the Fox Chase laboratories, illustrating the high demand for such technology and services at the Center.
The Cell Culture Facility also provides technical support, cells, reagents, and supplies for establishing germline transmissible embryonic stem (ES) cells for developing gene knockout mice. Seven different ES cell lines from three species of mice, as well as feeder layer cells (mouse embryo fibroblasts), and all necessary reagents are in stock for immediate use in the facility.
The Cell Culture Facility further aids Fox Chase investigators in the production of monoclonal antibodies to a wide variety of antigens. The Facility provides information on mouse immunization and antibody screening protocols, fuses immunized mouse, hamster or rat spleen cells with a drug-sensitive myeloma fusion partner, grows and isolates primary hybriodoma clones, provides culture supernatants for antibody assays, clones desired hybridomas, provides high-titer antibody-containing culture supernatants, purifies and labels monoclonal antibodies, and freezes representative cell samples for long-term viable storage in liquid nitrogen.
The Cell Culture Facility maintains a computer catalog that accounts for about 58,000 vials of cell lines banked within the facility. The computer database is accessible to Fox Chase researchers for searching via internal intranet access. This allows investigators to directly access their inventory of frozen stocks and improve the sharing of cell reagents among the researchers at Fox Chase.