Facility Services Table

The table can be sorted in alphabetical order by clicking on the blue arrows to the right of any column name.  For example, you can sort the table by title of the service or by name of the facility.  You can also view only services that contain a specific word or words by typing them in the Search box.  So you can find services that contain the word "cells" or "mice" for example.

The link on each facility name will take you to that facility's web page, and the links on email addresses will open an email message to that individual.

ServiceContactFacility NameLocation/Phone

Preparation of sterile growth media and reagents

The CCF is a cost-effective resource for the preparation of complex media as well as of the more common media.

Pamela Nakajima, PhD
Pamela.Nakajima@fccc.edu
Cell Culture Reimann R288
215-728-2486

Performance testing of fetal bovine serum

By testing and purchasing an optimal fetal bovine serum batch, investigators have access to a consistent, quality-assured supply of at the most cost‑effective price.

Pamela Nakajima, PhD
Pamela.Nakajima@fccc.edu
Cell Culture Reimann R288
215-728-2486

Cell propagation

Established cell lines are routinely obtained from a variety of sources and passaged in culture.

Pamela Nakajima, PhD
Pamela.Nakajima@fccc.edu
Cell Culture Reimann R288
215-728-2486

Initiation of cell lines

A wide array of established techniques is used to prepare primary cultures and to derive serially subcultured cell lines from them.

Pamela Nakajima, PhD
Pamela.Nakajima@fccc.edu
Cell Culture Reimann R288
215-728-2486

EBV transformation

Immortalized B cells can be generated from human peripheral blood samples by transformation with Epstein Barr Virus (EBV).

Pamela Nakajima, PhD
Pamela.Nakajima@fccc.edu
Cell Culture Reimann R288
215-728-2486

Mycoplasma screening

Mycoplasma infestations of mammalian cell cultures can be detected by transferring their medium to indicator cultures of Vero monkey kidney cells and detecting mycoplasma DNA by fluorescence microscopy.

Pamela Nakajima, PhD
Pamela.Nakajima@fccc.edu
Cell Culture Reimann R288
215-728-2486

Embryonic stem cell culture

The Facility provides technical support, cells, reagents, and supplies for culturing mouse embryonic stem (ES) cells, and performs transfections and cloning to establish germline transmissible modified mouse ES cells to create knockout or knock-in mice

Pamela Nakajima, PhD
Pamela.Nakajima@fccc.edu
Cell Culture Reimann R288
215-728-2486

Hybridoma fusions and antibody production

The Facility can produce hybridoma fusions, clone antibody-producing hybridomas, generate high titer monoclonal antibody (mAb)-containing culture supernatants, and bank hybridomas.

Pamela Nakajima, PhD
Pamela.Nakajima@fccc.edu
Cell Culture Reimann R288
215-728-2486

Frozen storage of viable cells (cell banking)

Viable cell samples are stored under liquid nitrogen (‑196°C) in cell banks with a total capacity of about 57,000 samples.

Pamela Nakajima, PhD
Pamela.Nakajima@fccc.edu
Cell Culture Reimann R288
215-728-2486

Cell Validation Support

The Facility supports FCCC researchers in validating the integrity of their cell lines by providing a wide array of common stock cell lines that were obtained directly from approved sources

Pamela Nakajima, PhD
Pamela.Nakajima@fccc.edu
Cell Culture Reimann R288
215-728-2486

Flow cytometry analysis

Cell surface staining using fluorescent-conjugated monoclonal antibodies can be analyzed.  Internal determinants can be analyzed if cells are first permeabilized prior to staining.

Richard R Hardy, PhD Cell Sorting Reimann R385
215-728-2469

Flow cytometry sorting

Cells with desired characteristics (distinctive protein expression, GFP+, etc.) can be isolated.

Richard R Hardy, PhD Cell Sorting Reimann R385
215-728-2469

Ca++ flux measurement

Measurement of Indo-1 loaded cells enables determination of intracellular calcium ion concentration and alteration after cell stimulation.

Richard R Hardy, PhD Cell Sorting Reimann R385
215-728-2469

Irradiation of samples

Samples (biochemical, cell culture or animal) are irradiated with specific doses.  Irradiations can be done to investigate radiobiologic mechanisms, to suppress immune response in animals or to sterilize cells or equipment.

Corinne Stobbe, MSc
Corinne.Stobbe@fccc.edu
Reimann R314
Irradiation* Reimann R314
215-728-4311

Planning and dosimetry

Facility staff are available for consultation on the planning of experiments, including the dosimetry for custom set-ups.

Corinne Stobbe, MSc
Corinne.Stobbe@fccc.edu
Reimann R314
Irradiation* Reimann R314
215-728-4311

Training

Training is provided in accordance with PA-DEP regulations for staff to become independent users of the irradiators.

Corinne Stobbe, MSc
Corinne.Stobbe@fccc.edu
Reimann R314
Irradiation* Reimann R314
215-728-4311

Tissue processing/paraffin blocks and sections

Preparation of histological sections from tissues provided by investigators

Catherine Renner
Catherine.Renner@fccc.edu
215-728-2464
Center C416
Histopathology Center C416
215-728-2464

Tissue sectioning from frozen blocks

Preparation  of histological cryosections from animal tissues provided by investigators

Catherine Renner
Catherine.Renner@fccc.edu
215-728-2464
Center C416
Histopathology Center C416
215-728-2464

Tissue section staining

H & E, and other classical histological techniques for microscopic evaluation of tissues and cells

Catherine Renner
Catherine.Renner@fccc.edu
215-728-2464
Center C416
Histopathology Center C416
215-728-2464

Immunohistochemistry

Detection of  cancer-related proteins in tissue sections

Catherine Renner
Catherine.Renner@fccc.edu
215-728-2464
Center C416
Histopathology Center C416
215-728-2464

Interpretative pathology

Pathologists of the Facility will read slides and report on the abnormalities found when requested.

Catherine Renner
Catherine.Renner@fccc.edu
215-728-2464
Center C416
Histopathology Center C416
215-728-2464

Histopathology Reports

Web based database with all the cases signed-out by the Facility Director.  Found here.

Catherine Renner
Catherine.Renner@fccc.edu
215-728-2464
Center C416
Histopathology Center C416
215-728-2464

Digital photography

Pathologists of the Facility will document microscopic findings when requested

Catherine Renner
Catherine.Renner@fccc.edu
215-728-2464
Center C416
Histopathology Center C416
215-728-2464

Image Analysis

Advanced computer-assisted image analyzers permit morphometric and densitometric analysis of tissue components and IHC reactions.

Catherine Renner
Catherine.Renner@fccc.edu
215-728-2464
Center C416
Histopathology Center C416
215-728-2464

Protein Characterization

• Characterization of recombinant DNA expressed proteins and partially purified proteins at high sensitivity.
• Protein identification from affinity pull-down experiments.
• Characterization of post-translational modifications.
• Identify protein internal cleavage sites.

Yibai Chen, PhD
Yibai.Chen@fccc.edu
215-728-3470
Reimann R150
Biochemistry and Biotechnology Reimann R150
215-728-3470

Metabolite/Drug Analytical Chemistry

• Quantitative and qualitative analysis of metabolites and small molecules.
• Compound identification.
• Quantitation of drug from plasma samples.

Yibai Chen, PhD
Yibai.Chen@fccc.edu
215-728-3470
Reimann R150
Biochemistry and Biotechnology Reimann R150
215-728-3470

Biomarker Discovery

• 2D gel comparative proteomics, high throughput protein identification by MALDI-TOF and using LC/MS/MS to identify complex samples.
• LC/MS/MS comparative proteomics with or without isotope labeling.
• GeLC/MS/MS comparative proteomics

Yibai Chen, PhD
Yibai.Chen@fccc.edu
215-728-3470
Reimann R150
Biochemistry and Biotechnology Reimann R150
215-728-3470

Protein Purification

• Fractionation of conditioned media, by size and by charge.
• Protein mixture purification.

Yibai Chen, PhD
Yibai.Chen@fccc.edu
215-728-3470
Reimann R150
Biochemistry and Biotechnology Reimann R150
215-728-3470

Methods development

• Hydrogen-deuterium exchange.
• Protein kinase substrate tagging methods.
• Quantitative analysis of new compounds using LC/MS/MS.

Yibai Chen, PhD
Yibai.Chen@fccc.edu
215-728-3470
Reimann R150
Biochemistry and Biotechnology Reimann R150
215-728-3470

Magnetic Resonance Micro-Imaging

High resolution anatomic imaging of tumors and other anatomical structures in mice.

Harvey Hensley, PhD
Harvey.Hensley@fccc.edu
215-728-3156
Reimann R464
Small Animal Imaging Reimann R464
215-728-3620

Bioluminescence and Fluorescence imaging

In vivo measurement of the signals from bioluminescent or fluorescence probes in mice.

Harvey Hensley, PhD
Harvey.Hensley@fccc.edu
215-728-3156
Reimann R464
Small Animal Imaging Reimann R464
215-728-3620

Fluorescence Molecular Tomography

In-vivo quantitative reconstruction in  three dimensions of the distribution of biologically targeted infrared fluorphores.

Harvey Hensley, PhD
Harvey.Hensley@fccc.edu
215-728-3156
Reimann R464
Small Animal Imaging Reimann R464
215-728-3620

Mouse Endoscopy

Provides for the detection and serial imaging of polyps in a mouse model for colon cancer.

Harvey Hensley, PhD
Harvey.Hensley@fccc.edu
215-728-3156
Reimann R464
Small Animal Imaging Reimann R464
215-728-3620

Human Functional Brain Imaging

Measure the regions of activation in the human brain when performing specific tasks. Used for behavioral studies.

Harvey Hensley, PhD
Harvey.Hensley@fccc.edu
215-728-3156
Reimann R464
Small Animal Imaging Reimann R464
215-728-3620

Assay Development

The facility assists investigators in the development of robust assays for both basic science applications and prior to initiating screening.

Margret Einarson, PhD
Margret.Einarson@fccc.edu
215-728-2542
West
High Throughput Screening West W250 & 251
215-728-2542

Screening

The facility supports siRNA and small molecule screening by providing reagents, assay development support, training on instrumentation, and support during screen execution.

Margret Einarson, PhD
Margret.Einarson@fccc.edu
215-728-2542
West
High Throughput Screening West W250 & 251
215-728-2542

Libraries Available for Screening

-RNAi library:
Thermo Fisher Dharmacon whole genome siRNA library comprising siRNA SMARTpools targeting approximately 22,000 genes.

-Small molecule libraries:
ChemDiv Diverse Collection – 50,000 compounds

-ICCB known Bioactives -480 compounds (Enzo Life Sciences; http://www.enzolifesciences.com/BML-2840/iccb-known-bioactives-library/)

Margret Einarson, PhD
Margret.Einarson@fccc.edu
215-728-2542
West
High Throughput Screening West W250 & 251
215-728-2542

Clinical Correlates

The facility develops and executes clinical correlates at the request of investigators.  Please contact Margret Einarson  x. 215-728-2542 for additional information.

Margret Einarson, PhD
Margret.Einarson@fccc.edu
215-728-2542
West
High Throughput Screening West W250 & 251
215-728-2542

Negative staining (for TEM)

Provides simple way of imaging of protein molecules, macromolecular complexes, viral particles and isolated organels.

Andrey Efimov, PhD
andrey.efimov@fccc.edu
215-728-5675
Reimann R379
Electron Microscopy Reimann R379
215-728-5675

Nucleic acid preparation (for TEM)

DNA or RNA molecules are spread on the suface of aqueous buffer either free or in a monolayer of denatured protein. Upon attachment to carbon-coated film, the molecules are contrasted by rotary shadowing with heavy metal.

Andrey Efimov, PhD
andrey.efimov@fccc.edu
215-728-5675
Reimann R379
Electron Microscopy Reimann R379
215-728-5675

Epoxy embedding (for TEM)

Standard way of looking at the ultrastructure of cultured cells or tissues. Also can be used in conjunction with pre-embedding immunolabeling.

Andrey Efimov, PhD
andrey.efimov@fccc.edu
215-728-5675
Reimann R379
Electron Microscopy Reimann R379
215-728-5675

Sample preparation and imaging in SEM

SEM is used to study the surface of the samples of interest. The object has to be first chemically fixed, dehydrated by critical point drying and further made electrically conductive by sputtering with platinum.

Andrey Efimov, PhD
andrey.efimov@fccc.edu
215-728-5675
Reimann R379
Electron Microscopy Reimann R379
215-728-5675

Cryosectioning and immuncytochemistry on frozen sections

Samples of cell pelets or tissues are lightly chemically fixed and cryoprotected with sucrose. Upon freezing in liquid nitrogen, thin sections are cut at low temperature (-120°C) and labeled by indirect immunocytochemistry using gold particles as the electrondense marker (Tokuyasu method).

Andrey Efimov, PhD
andrey.efimov@fccc.edu
215-728-5675
Reimann R379
Electron Microscopy Reimann R379
215-728-5675

Training, consultation, presentation of results

The Facility mostly provides experiment planning, all or most of the sample preparation and imaging. Help is also available with image processing and preparation for presentation.

Andrey Efimov, PhD
andrey.efimov@fccc.edu
215-728-5675
Reimann R379
Electron Microscopy Reimann R379
215-728-5675

Training

The Facility requires that all users that wish to work on the Facility's instruments are trained by the Staff.

Andrey Efimov, PhD
andrey.efimov@fccc.edu
215-728-5675
Reimann R379
Light Microscopy Reimann R379
215-728-5675

Consultations and Troubleshooting

The users are encouraged to discuss their imaging needs with the Staff so that optimal sample preparation protocol, imaging mode and image processing can be selected and tuned for the particular application.

Andrey Efimov, PhD
andrey.efimov@fccc.edu
215-728-5675
Reimann R379
Light Microscopy Reimann R379
215-728-5675

Chromosome painting using HiSKY spectral imaging

The recently acquired ASI  spectral head for Nikon Eclipse 800 microscope enables to map chromosomes in spreads by "chromosome painting" technology based on hybridization with fuorescent probes and subsequent spectral imaging and image processing.

Andrey Efimov, PhD
andrey.efimov@fccc.edu
215-728-5675
Reimann R379
Light Microscopy Reimann R379
215-728-5675

Semithin cryosection preparation

Cryosamples of tissues and cell pellets are prepared using Tokuyasu method and semi-thin sections cut on a Leica cryotome.

Andrey Efimov, PhD
andrey.efimov@fccc.edu
215-728-5675
Reimann R379
Light Microscopy Reimann R379
215-728-5675

Microinjection

The facility is equipped with an Eppendorf microinjector that is used for delivery of plasmids to cultured cells.

Andrey Efimov, PhD
andrey.efimov@fccc.edu
215-728-5675
Reimann R379
Light Microscopy Reimann R379
215-728-5675

DNA dye terminator sequence analysis

User supplied DNA templates (plasmids, PCR product) are analyzed to determine DNA sequence, typically generating over 700 bases of data.

Richard R Hardy, PhD DNA Sequencing Reimann R382
215-728-2469

Cytogenetic analysis of human, mouse and rat chromosomes

Cell line species verification; computation of modal chromosome number; karyotypic analysis of blood, bone marrow, solid tumors, and cell lines; mapping the location of viral or transgene integrations, identification of chromosomal breakpoints, and detection of amplified or deleted genes in human, rat, and mouse metaphases by fluorescence in situ hybridization (FISH); interphase FISH; and chromosome painting and multiplex-FISH (M-FISH) to facilitate the interpretation of chromosome rearrangements.

no contact listed Cytogenetics and Chromosome Microarray Analysis CPP P3011
215-728-2898

DNA copy number analysis; genomic analysis

Utilization of Affymetrix platform for various DNA copy number and loss of heterozygosity (LOH) analyses, pharmacogenetics, and promoter methylation studies.  SNP analysis for GWAS, using the Affymetrix platform, and array-CGH analysis of murine or human samples, using Agilent's DNA microarray-based technology.

no contact listed Cytogenetics and Chromosome Microarray Analysis CPP P3011
215-728-2898

Microarray-based RNA and microRNA(miRNA) expression analysis

The DNA Microarray Core provides full microarray hybridization services, including RNA isolation, probe labeling, hybridization, scanning and data extraction.  The raw data is then uploaded into the caArray database system for storage and management.  Users receive quantified microarray data sheets ready for further analysis.  This Core coordinates with the Biostatistics and Informatics Facility for microarray data mining and management.

MicroRNAs (miRNAs) have recently emerged as crucial regulator of gene expression, development, proliferation and differentiation, and the this Core provides microarray technology to miRNA expression analysis, thereby permitting sensitive and accurate global miRNA profiling.

no contact listed Expression Microarray CPP P3016
215-728-3174

Genotyping; real-time PCR (RT-PCR)

This Core of the Genomics Facility performs cost-effective mouse genotyping services and qRT-PCR.  For genotyping, the Core performs DNA extraction from mouse tails and toe clips and develops, optimizes and performs allele-specific PCR assays for the genotyping of knockout, knock-in, and transgenic mouse strains. This service is carried out in collaboration with the Laboratory Animal Facility (LAF) with communication through the on-line mouse genotyping database that tracks the matings, genotyping results and animal inventory for each strain of mice.  The Core also offers HLA genotyping service for human samples.

Quantitative RT-PCR is offered for various purposes, including validation of DNA array gene expression data, quantification of gene expression, quantification of virus load, zygosity determination, quantitative analysis of alternative spliced transcripts, quantification of gene-specific DNA in immunoprecipitated ChIP assays, and validation of the effectiveness of siRNA transcription silencing.  The Core is equipped with several complementary technology platforms that allow for comprehensive and cost-effective analysis of variations and alterations such as SNPs, mutations, gene deletions, variable nucleotide tandem repeats, LOH, and CpG methylation.  These platforms include pyrosequencing (PSQ96MA, Biotage), capillary electrophoresis (CEQ8800, Beckman Coulter) and two real-time fluorescent PCR machines (ABI PRISM 7900 SDS, Applied Biosystems) in a 96-well, 384-well or low-density array format.

no contact listed Genotyping and Real-Time PCR CPP P3013
215-728-2489 (Manager); 215-214-1696 (Staff)

Laser capture microdissection (LCM)

Instrumentation and expertise needed for the isolation of pure populations of cells from heterogeneous tissue specimens.  LCM services include identification and isolation of tumor cells or other cells of interest, as well as DNA, RNA and protein purification from LCM samples.  This Core is equipped with two LCM systems (PixCell II® and Veritas™, Molecular Devices), which are available for a wide range of applications, including LCM of specific immunostained or fluorescent cells. A SYBR Green assay is utilized to screen formalin-fixed paraffin-embedded (FFPE) tissue blocks for intact RNA that is suitable for microarray or RT-PCR analysis.  Amplification of limited quantities of total RNA from microdissected frozen and FFPE tissues is also available.

no contact listed Laser Capture Microdissection CPP P3011
215-728-2898