Faculty Summaries
Erica A. Golemis, PhD
Erica A. Golemis, PhD
  • Deputy Chief Scientific Officer
  • Co-Leader, Molecular Therapeutics
Office Phone: 215-728-2860
Lab Phone: 215-728-3885
Fax: 215-728-3616
Office: W406

Research Articles

  • Plimack, E., R., Dunbrack, R.L., Brennan, T., Andrake, M, Zhou, Y., Serebriiskii, I.G., Wei, Q., Slifker, M., Alpaugh, K., Dulaimi, E., Palma, N., Hoffman-Censits, J., Wong, Y.-N., Bilusic, M., Kutikov, A., Viterbo, R., Greenberg, R.E., Chen, D., Lallas, C.D., Trabulsi, E.J., Yelensky, R., McConkey, D., Miller, V.A., Golemis, E.A., and Ross, E.G. Alterations in ATM, Rb1 or FANCC predict response to neoadjuvant cisplatin based chemotherapy in muscle invasive bladder cancer. Europ Urol, Epub July 31 2015. PubMed
  • Shagisultanova, E., Dunbrack Jr., R.L., and Golemis, E.A. Issues in interpreting the in vivo activity of Aurora-A. Exp Opin Ther Targets, in press.
  • Beck, T.N., Gabitova, L., and I.G. Serebriiskii. “Targeted Therapy: Genomics Approaches.” In R. Meyers (Ed.), Encyclopedia of Molecular Cell Biology and Molecular Medicine. Weinheim: Wiley-Blackwell. 2014, in press.
  • Boquoi A, Arora S, Chen T, Litwin S, Koh J, and Enders GH. Reversible cell cycle inhibition and premature aging imposed by conditional expression of p16Ink4a. Aging Cell – In press.
  • Little, J.L., Serzhanova, V., Izumchenko, E., Egleston, B.L., Parise, E., Klein-Szanto, A.J., Loudon, G., Shubina, M., Seo, S., Kurokawa, M., Ochs, M.F., and Golemis, E.A. A requirement for Nedd9 in luminal progenitor cells prior to mammary tumorigenesis in MMTV-HER2/ErbB2 mice. Oncogene, 33:411-420, 2014. PubMed
  • Purdy AK, Alvarez Arias DA, Oshinsky J, James AM, Serebriiskii I, Campbell KS. The ap-2 clathrin adaptor mediates endocytosis of an inhibitory killer cell Ig-like receptor in human NK cells. J. Immun. 193(9):4675-83, 2014.
  • Do, T.-V., Bickel, L.E., Klein-Szanto, A.J., Pathak, H.B., Hua, X., Howe, C., Xiao, F., O’Brien, S., Maglaty, M., Ecsedy, J.A., Litwin, S., Golemis, E.A., Schilder, R.J., Godwin, A.K., and Connolly, D.C. Aurora kinase A mediates epithelial ovarian cancer migration and adhesion. Oncogene 33:539-549, 2014.
  • Nikonova, A.S., Serzhanova, V., Plotnikova, O.V., Efimov, A., Bogush, I., Cai, K.Q., Egleston, B.L., Klein-Szanto, A.S., Seeger-Nukpezah, T.N., and Golemis, E.A. Nedd9 restrains renal cystogenesis in Pkd1-/- mice.  Proc Nat Acad Sci 111:12859-64, 2014. PubMed
  • Liu, H., Cracchiolo, J.R., Beck, T.N., Serebriiskii, I.G., and Golemis, E.A. EGFR inhibitors as therapeutic agents in head and neck cancer. in Molecular Determinants of Head and Neck Cancer, eds, Burtness B. and Golemis E.A., Current Cancer Research, Springer Press, pp 55-90, 2014.
  • Liu, H., Beck, T.N., Golemis, E.A., and Serebriiskii, I.G. Integrating in silico resources to map a signaling network. Methods in Mol Biol. 1101:197-245, 2014.
  • Beck, T. N., Chikwem, A.J., Solanki, N.R., and Golemis, E.A. Bioinformatic Approaches to Augment Study of Epithelial-to-Mesenchymal Transition (EMT) in Lung Cancer. Physiol Genomics 46:699-724, 2014.
  • Beck, T.N., Nicolas, E., Kopp, M.C., and Golemis, E.A. Adaptors for disorders of the brain? The cancer signaling proteins NEDD9, CASS4, and PTK2B in Alzheimer’s Disease. Oncoscience 1(7):486-503, 2014.
  • Nikonova, A.S., Gaponova, A.V., Kudinov, A.E., and Golemis, E.A. CAS proteins in health and disease: an update. IUBMB Life 66:387-95, 2014.
  • Sukhanova, A., Gorin, A., Serebriiskii, I.G., Gabitova, L., Zheng, H., Restifo, D., Egleston, B., Cunningham, D., Bagnyukova, T., Liu, H., Nikonova, A., Adams, G.P., Zhou, Y., Yang, D., Mehra, R., Burtness, B., Cai, K.Q., Klein-Szanto, A., Kratz, L.E., Kelley, R.I., Weiner, L.M., Herman, G.E., Golemis, E.A., and Astsaturov, I. Targeting C4-demethylating genes in the cholesterol pathway sensitizes cancer cells to EGFR inhibitors via increased EGFR degradation. Cancer Discovery, Jan;3(1):96-111. doi: 10.1158/2159-8290.CD-12-0031, 2013.
  • Seeger-Nukpezah, T., Proia, D.A., Egleston, B.L., Nikonova, A.S., Kent, T., Cai, K.Q., Hensley, H.H., Ying, W., Chimmanamada, D., Serebriiskii, I.G., and Golemis, E.A. A novel Hsp90 inhibitor slows cyst growth in a mouse model of autosomal dominant polycystic kidney disease (ADPKD). Proc. Nat. Acad Sci. 110:12786-91, 2013.
  • Bagnyukova, T.V., Restifo, D., Beeharry, N., Gabitova, L., Li, T., Serebriiskii, I.G., Golemis, E.A., and Astsaturov, I. DUSP6 regulates drug sensitivity by modulating DNA damage response. Br J Cancer. 109(4):1063-71, 2013.
  • Liu, H., Xiao, F., Serebriiskii, I.G., O’Brien, S.W., Maglaty, M.A., Astsaturov, I.A., Litwin, S., Martin, L.P., Proia, D.A., Golemis, E.A.* and Connolly, D.C.* Network analysis identifies an HSP90-central hub susceptible in ovarian cancer. Clin Cancer Res, 19:5053-5057, 2013.
  • Mehra, R., Zhu, F., Yang, D.-H., Cai, K.Q., Weaver J., Singh, M.K., Nikonova, A.S., Golemis, E.A., Flieder, D.B., Cooper, H.S., Lango, M., Ridge, J.A., and Burtness, B. Quantification of excision repair cross complementing group 1 (ERCC1) and survival in squamous cell head and neck cancers. Clin Cancer Res 19:6633-43, 2013.
  • Mehra, R., Serebriiskii, I.G., Burtness, B., Astsaturov, I., and Golemis, E. The role and targeting of Aurora kinases in head and neck cancer. Lancet Oncology 14:e425-435, 2013.
  • Khazak, V., Eyrisch, S., Kato, J., Tamanoi, F., and Golemis, E.A. A two-hybrid approach to identify inhibitors of the RAS-RAF interaction. The Enzymes vol 33: Chapter 10 in Inhibitors of the Ras Superfamily G-proteins. Academic Press, Elsevier, pp 214-248, 2013.
  • Seeger-Nukpezah, T., Little, J.L., Serzhanova, V., and Golemis, E.A. Cilia and cilia-associated proteins in cancer. Drug Discov Today: Dis. Mech. 10:e135-142, 2013.
  • Little, JL, Golemis, EA. NEDD9 (HEF1, CAS-L). Encyclopedia of Cancer Targets, SpringerReference 2013.
  • Nikonova, A.S., Astsaturov, I., Serebriiskii, I.G., Dunbrack, R.L. Jr., and Golemis, E.A. Aurora-A kinase (AURKA) in normal and pathological cell division. Cell Mol Life Sci. 70(4):661-87, 2013.
  • Plotnikova, O.V., Nikonova, A.S., Pugacheva, E.N., and Golemis, E.A. Calmodulin activation of Aurora-A (AURKA) is required during ciliary disassembly and in mitosis. Mol Biol Cell 2012 Jul;23(14):2658-70. PMCID:PMC3395655
  • Pan, J., Seeger-Nukpezah, T., and Golemis, E.A. The role of the cilium in normal and abnormal cell cycles: emphasis on renal cystic pathologies. Cell Mol Life Sci, 70(11):1849-74, 2013.
  • Burtness, B., Marur, S., Bauman, J.E., Golemis, E.A., Mehra, R., and Cohen, S.J. Comment on Epidermal Growth Factor Receptor Is Essential for Toll-Like Receptor 3 Signaling. Sci. Signal. 5, lc5, 2012.
  • Seeger-Nukpezah, T., and Golemis, E.A. The extracellular matrix and ciliary signaling. Current Opin Cell Biol 24, Epub July 19 2012.
  • Seeger-Nukpezah T, Liebau MC, Hopker K, Lamkemeyer T, Benzing T, Golemis EA, Schermer B. The centrosomal kinase plk1 localizes to the transition zone of primary cilia and induces phosphorylation of nephrocystin-1. PLoS One. 2012 Jan;7(6):e38838. PMCID: PMC3372538
  • Dotan E, Meropol NJ, Zhu F, Zambito F, Bove B, Cai KQ, Godwin AK, Golemis EA, Astsaturov I, Cohen SJ. Relationship of increased aurora kinase A gene copy number, prognosis and response to chemotherapy in patients with metastatic colorectal cancer. Br J Cancer. 2012 Feb;106(4):748-55. PMCID: PMC3322945
  • Mehra R, Serebriiskii IG, Dunbrack RL, Robinson MK, Burtness B, Golemis EA. Protein-intrinsic and signaling network-based sources of resistance to EGFR- and ErbB family-targeted therapies in head and neck cancer. Drug Resistance Updates. 2011 Dec;14(6):260-79. PMCID: PMC3195944
  • Ratushny V, Pathak HB, Beeharry N, Tikhmyanova N, Xiao F, Li T, Litwin S, Connolly DC, Yen TJ, Weiner LM, Godwin AK, Golemis EA. Dual inhibition of SRC and Aurora kinases induces postmitotic attachment defects and cell death. Oncogene. 2012 Mar 08;31(10):1217-27. PMCID: PMC3204164. Tikhmyanova N, Golemis EA. NEDD9 and BCAR1 Negatively Regulate E-Cadherin Membrane Localization, and Promote E-Cadherin Degradation. PLoS One. 2011 Jan;6(7):e22102. PMCID: PMC3134485
  • Plotnikova, O.V., Pugacheva, E.P., and Golemis, E.A., Aurora-A kinase activity influences calcium signaling in kidney cells. J. Cell Biol 193:1021-1032, 2011.
  • Li, Y., Bavarva, J.H., Wang, Z., Guo, J. Qian, C., Thibodeau, S.N., Golemis, E.A., and Liu, W. HEF1, a novel target of Wnt signaling, promotes colonic cell migration and cancer progression. Oncogene 30:2633-43, 2011.
  • Singh, M.K., Izumchenko, E., Klein-Szanto, A., Egleston, B.L., Wolfson, M., Golemis, E.A. Enhanced genetic instability and dasatinib sensitivity in mammary tumor cells lacking Nedd9. Cancer Research 70:8907-8916, 2010.
  • Moore, F.E., Osmundson, E.C., Koblinski, J., Pugacheva, E., Golemis, E.A., Ray, D., and Kiyokawa, H. The WW-HECT protein Smurf2 interacts with the docking protein Nedd9/HEF1 for Aurora A activation. Cell Division 5:22, 2010.
  • Astsaturov I, Ratushny V, Sukhanova A, Einarson M, Bagnyukova T, Zhou Y, Devarajan K, Silverman JS, Tikhmyanova N, Skobeleva N, Pecherskaya A, Nasto R, Jablonski SJ, Serebriiskii IG, Weiner LM, and Golemis EA. Synthetic lethal screen of an EGFR-centered signaling network defines clusters of proteins contributing to drug resistance. Science Signaling, 3(140):ra67, 2010.
  • Tikhmyanova N, Tulin A, Roegiers F, and Golemis EA. Dcas supports cell polarization and cell-cell adhesion complexes in development. PLoS One. 2010 Aug 24;5(8).
  • Plotnikova OV, Pugacheva EN, Dunbrack RL, Jr., and Golemis EA. Rapid calcium-dependent activation of Aurora-A kinase. Nature Commun. 1 : 64 doi: 10.1038/ncomms1061 Epub September 7, 2010.
  • Singh MK, Izumchenko E, Klein-Szanto A, Egleston BL, Wolfson M, Golemis EA. Enhanced genetic instability and dasatinib sensitivity in mammary tumor cells lacking Nedd9. Cancer Res, 2010 in press.

Research Articles 2000-2009

  • Izumchenko E, Singh MK, Plotnikova OV, Tikhmyanova N, Little JL, Serebriiskii IG, Seo S, Kurokawa M, Egleston BL, Klein-Szanto A, Pugacheva EN, Hardy RR, Wolfson M, Connolly DC, Golemis EA. HEF1/CAS-L/NEDD9 null status limits mouse mammary tumor progression. Cancer Research. 61:7198-206, 2009.
  • Aquino JB, Lallemend F, Marmigere F, Adameyko, Golemis EA, Ernfors P. The retinoic acid inducible Cas-Family signaling protein Nedd9 regulates neural crest cell migration by modulating adhesion and actin dynamics. Neuroscience. 2009 Sep;162(4):1106-19.
  • Serebriiskii I, Castello-Cros R, Lamb A, Golemis EA, Cukierman E. Fibroblast-derived 3D matrix differentially regulates the growth and drug-responsiveness of human cancer cells. Matrix Biol. 2008 Jul;27(6):573-85.
  • Singh MK, Dadke D, Nicolas E, Serebriiskii IG, Apostolou S, Canutescu A, Egleston BL, and Golemis EA. A novel Cas-family member, HEPL, regulates FAK and cell spreading. Mol Biol Cell, 19:1627-1636, 2008.
  • Snell WJ, Golemis EA. A ciliary timer for S-phase entry. Nature Reviews Molecular Cell Biology. 2007 Sep;.
  • Pugacheva EN, Jablonski SA, Hartman TR, Henske EP, Golemis EA. HEF1-dependent Aurora A activation induces disassembly of the primary cilium.[see comment]. Cell. 2007 Jun 29;129(7):1351-63.
  • Skobeleva N, Menon S, Weber L, Golemis EA, Khazak V. In vitro and in vivo synergy of MCP compounds with mitogen-activated protein kinase pathway- and microtubule-targeting inhibitors. Mol Cancer Ther. 2007 Mar;6(3):898-906.
  • Lee H, Kim D, Dan HC, Wu EL, Gritsko TM, Cao CH, Nicosia SV, Golemis EA, Liu WG, Coppola D, Brem SS, Testa JR, Cheng JQ. Identification and characterization of putative tumor suppressor NGB, a GTP-binding protein that interacts with the neurofibromatosis 2 protein. Mol Cell Biol. 2007 Mar;27(6):2103-19.
  • Singh MK, Nicolas E, Gherraby W, Dadke D, Lessin S, Golemis EA. HEI10 negatively regulates cell invasion by inhibiting cyclin B/Cdk1 and other promotility proteins. Oncogene. 2007 Jul 19;26(33):4825-32.
  • Gronholm M, Muranen T, Toby GG, Utermark T, Hanemann CO, Golemis EA, Carpen O. A functional association between merlin and HEI10, a cell cycle regulator. Oncogene. 2006 Jul;25(32):4389-98.
  • 62. Dadke D, Jarnik M, Pugacheva EN, Singh MK, Golemis EA. Deregulation of HEF1 impairs M-phase progression by disrupting the RhoA activation cycle. Mol Biol Cell. 2006;17:1204-17.
  • Pugacheva EN, Golemis EA. HEF1-Aurora A interactions - Points of dialog between the cell cycle and cell attachment signaling networks. CELL CYCLE. 2006 Feb;5(4):384-91.
  • Natarajan M, Stewart JE, Golemis EA, Pugacheva EN, Alexandropoulos K, Cox BD, Wang W, Grammer JR, Gladson CL. HEF1 is a necessary and specific downstream effector of FAK that promotes the migration of glioblastoma cells. Oncogene. 2006 Mar;25(12):1721-32.
  • Pugacheva EN, Golemis EA. HEF1 regulates centrosomal maturation and spindle formation through control of the Aurora A kinase. Nature Cell Biol. 2005;7:937-46.
  • Jin T, Howard A, Golemis EA, Wang Y, Zhang Y-Z. Overproduction, purification, crystallization and preliminary X-ray diffraction studies of the human transcription repressor ERH Acta Cryst F61. 531-533, 2005.
  • Serebriiskii IG, Fang R, Latypova E, Hopkins R, Vinson C, Joung JK, Golemis EA. A combined yeast/bacteria two-hybrid system - Development and evaluation. Mol Cell Proteomics. 2005 Jun;4(6):819-26.
  • Jin T, Howard AJ, Golemis EA, Wang Y, Zhang YZ. Overproduction, purification, crystallization and preliminary X-ray diffraction studies of the human spliceosomal protein TXNL4B. Acta Crystallographica Section F-Structural Biology and Crystallization Communications. 2005 Part 3 MAR;61:282-4.
  • Sudomoina M, Latypova E, Favorova OO, Golemis EA, Serebriiskii IG. A gene expression system offering multiple levels of regulation: the Dual Drug Control (DDC) system. BMC Biotechnol. 2004 Apr 29;4:9.
  • Einarson MB, Cukierman E, Compton DA, Golemis EA. Human enhancer of invasion-cluster, a coiled-coil protein required for passage through mitosis. Mol Cell Biol. 2004 May;24(9):3957-71.
  • Cotsiki M, Lock RL, Cheng Y, Williams GL, Zhao J, Perera D, Freire R, Entwistle A, Golemis EA, Roberts TM, Jat PS, Gjoerup OV. Simian virus 40 large T antigen targets the spindle assembly checkpoint protein Bub1. Proc Natl Acad Sci U S A. 2004 Jan;101(4):947-52.
  • Dadke D, Fryer BH, Golemis EA, Field J. Activation of p21-activated kinase 1-nuclear factor kappaB signaling by Kaposi's sarcoma-associated herpes virus G protein-coupled receptor during cellular transformation. Cancer Res. 2003 Dec 15;63(24):8837-47.
  • Na X, Duan HO, Messing EM, Schoen SR, Ryan CK, di Sant'Agnese PA, Golemis EA, Wu G. Identification of the RNA polymerase II subunit hsRPB7 as a novel target of the von Hippel-Lindau protein. EMBO J. 2003 Aug 15;22(16):4249-59.
  • Zhang YZ, Cheng H, Gould KL, Golemis EA, Roder H. Structure, stability, and function of hDim1 investigated by NMR, circular dichroism, and mutational analysis. Biochemistry. 2003 Aug 19;42(32):9609-18.
  • Toby GG, Gherraby W, Coleman TR, Golemis EA. A Novel RING Finger Protein, Human Enhancer of Invasion 10, Alters Mitotic Progression through Regulation of Cyclin B Levels. Mol Cell Biol. 2003 Mar;23(6):2109-22.
  • Cai D, Felekkis KN, Near RI, O'Neill GM, van Seventer JM, Golemis EA, Lerner A. The GDP exchange factor AND-34 is expressed in B cells, associates with HEF1, and activates Cdc42. J Immunol. 2003 Jan 15;170(2):969-78.
  • Kato-Stankiewicz J, Hakimi I, Zhi G, Zhang J, Serebriiskii I, Guo L, Edamatsu H, Koide H, Menon S, Eckl R, Sakamuri S, Lu YC, Chen QZ, Agarwal S, Baumbach WR, Golemis EA, Tamanoi F, Khazak V. Inhibitors of Ras/Raf-1 interaction identified by two-hybrid screening revert Ras-dependent transformation phenotypes in human cancer cells. Proc Natl Acad Sci U S A. 2002 Oct 29 2002;99(22):14398-403.
  • Serebriiskii IG, Mitina O, Pugacheva EN, Benevolenskaya E, Kotova E, Toby GG, Khazak V, Kaelin WG, Chernoff J, Golemis EA. Detection of peptides, proteins, and drugs that selectively interact with protein targets. Genome Res. 2002 Nov;12(11):1785-91.
  • Zawistowski JS, Serebriiskii IG, Lee MF, Golemis EA, Marchuk DA. KRIT1 association with the integrin-binding protein ICAP-1: a new direction in the elucidation of cerebral cavernous malformations (CCM1) pathogenesis. Hum Mol Genet. 2002 Feb 15;11(4):389-96.
  • Fashena SJ, Einarson MB, O'Neill GM, Patriotis C, Golemis EA. Dissection of HEF1-dependent functions in motility and transcriptional regulation. J Cell Sci. 2002 Jan 1;115(Pt 1):99-111.
  • Reeder MK, Serebriiskii IG, Golemis EA, Chernoff J. Analysis of small GTPase signaling pathways using p21-activated kinase mutants that selectively couple to Cdc42. J Biol Chem. 2001 Nov 2;276(44):40606-13.
  • Serebriiskii IG, Mitina OV, Chernoff J, Golemis EA. Two-hybrid dual bait system to discriminate specificity of protein interactions in small GTPases. Methods Enzymol. 2001;332:277-300.
  • O'Neill GM, Golemis EA. Proteolysis of the docking protein HEF1 and implications for focal adhesion dynamics. Mol Cell Biol. 2001 Aug;21(15):5094-108.
  • van Seventer GA, Salmen HJ, Law SF, O'Neill GM, Mullen MM, Franz AM, Kanner SB, Golemis EA, van Seventer JM. Focal adhesion kinase regulates beta1 integrin-dependent T cell migration through an HEF1 effector pathway. Eur J Immunol. 2001 May;31(5):1417-27.
  • Sahoo T, Goenaga-Diaz E, Serebriiskii IG, Thomas JW, Kotova E, Cuellar JG, Peloquin JM, Golemis E, Beitinjaneh F, Green ED, Johnson EW, Marchuk DA. Computational and experimental analyses reveal previously undetected coding exons of the KRIT1 (CCM1) gene. Genomics. 2001 Jan 1;71(1):123-6.
  • Liu XH, Elia AE, Law SF, Golemis EA, Farley J, Wang TW. A novel ability of Smad3 to regulate proteasomal degradation of a Cas family member HEF1. EMBO J. 2000 Dec 15;19(24):6759-69.
  • Zhang Y, Lindblom T, Chang A, Sudol M, Sluder AE, Golemis EA. Evidence that dim1 associates with proteins involved in pre-mRNA splicing, and delineation of residues essential for dim1 interactions with hnRNP F and Npw38/PQBP-1. Gene. 2000 Oct 17;257(1):33-43.
  • Serebriiskii IG, Golemis EA. Uses of lacZ to study gene function: evaluation of beta-galactosidase assays employed in the yeast two-hybrid system. Anal Biochem. 2000 Oct 1;285(1):1-15.
  • Serebriiskii IG, Toby GG, Golemis EA. Streamlined yeast colorimetric reporter activity assays using scanners and plate readers. Biotechniques. 2000 Aug;29(2):278-9, 282-4, 286-8.
  • Law SF, O'Neill GM, Fashena SJ, Einarson MB, Golemis EA. The docking protein HEF1 is an apoptotic mediator at focal adhesion sites. Mol Cell Biol. 2000 Jul;20(14):5184-95.
  • Fashena SJ, Serebriiskii IG, Golemis EA. The continued evolution of hybrid screening approaches in yeast: How to outwit different preys with different baits. Gene. 2000;250:1-14.
  • Serebriiskii I, Estojak J, Berman M, Golemis EA. Approaches to detecting false positives in yeast two-hybrid systems. Biotechniques. 2000 Feb;28(2):328-30, 332-6.

Research Articles 1987-1999

  • Schaller S, Grandemange S, Shpakovski GV, Golemis EA, Kedinger C, Vigneron M. Interactions between the full complement of human RNA polymerase II subunits. FEBS Lett. 1999 Nov 19;461(3):253-7.
  • Zhang YZ, Gould KL, Dunbrack RJ, Cheng H, Roder H, Golemis EA. The evolutionarily conserved Dim1 protein defines a novel branch of the thioredoxin fold superfamily. Physiol Genomics. 1999 Nov 11;1(3):109-18.
  • Law SF, Zhang YZ, Fashena SJ, Toby G, Estojak J, Golemis EA. Dimerization of the docking/adaptor protein HEF1 via a carboxy- terminal helix-loop-helix domain. Exp Cell Res. 1999 Oct 10;252(1):224-35.
  • Mitsuuchi Y, Johnson SW, Sonoda G,Tanno S, Golemis EA, Testa JR. Identification of a chromosome 3p14.3-21.1 gene, APPL, encoding an adaptor molecule that interacts with the oncoprotein-serine/threonine kinase AKT2. Oncogene. 1999;18:4891-8.
  • Shen XQ, Bubulya A, Zhou XF, Khazak V, Golemis EA, Shemshedini L. Ligand-free RAR can interact with the RNA polymerase II subunit hsRPB7 and repress transcription. Endocrine. 1999 Jun;10(3):281-9.
  • Serebriiskii I, Khazak V, Golemis EA. A two-hybrid dual bait system to discriminate specificity of protein interactions. J Biol Chem. 1999 Jun 11;274(24):17080-7.
  • Lin JH, Makris A, McMahon C, Bear SE, Patriotis C, Prasad VR, Brent R, Golemis EA, Tsichlis PN. The ankyrin repeat-containing adaptor protein Tvl-1 is a novel substrate and regulator of Raf-1. J Biol Chem. 1999 May 21;274(21):14706-15.
  • Bellacosa A, Cicchillitti L, Schepis F, Riccio A, Yeung AT, Matsumoto Y, Golemis EA, Genuardi M, Neri G. MED1, a novel human methyl-CpG-binding endonuclease, interacts with DNA mismatch repair protein MLH1. Proc Natl Acad Sci U S A. 1999 Mar 30;96(7):3969-74.
  • Petermann R, Mossier BM, Aryee DN, Khazak V, Golemis EA, Kovar H. Oncogenic EWS-Fli1 interacts with hsRPB7, a subunit of human RNA polymerase II. Oncogene. 1998 Aug 6;17(5):603-10.
  • Law SF, Zhang YZ, Klein-Szanto AJ, Golemis EA. Cell cycle-regulated processing of HEF1 to multiple protein forms differentially targeted to multiple subcellular compartments. Mol Cell Biol. 1998 Jun;18(6):3540-51.
  • Toby G, Law SF, Golemis EA. Vectors to target protein domains to different cellular compartments. Biotechniques. 1998 Apr;24(4):637-40.
  • Khazak V, Estojak J, Cho H, Majors J, Sonoda G, Testa JR, Golemis EA. Analysis of the interaction of the novel RNA polymerase II (pol II) subunit hsRPB4 with its partner hsRPB7 and with pol II. Mol Cell Biol. 1998 Apr;18(4):1935-45.
  • 19. Astier A, Manie SN, Law S, Canty T, Hagheyeghi N, Druker BJ, Salgia R, Golemis EA, Freedman AS. Association of the Cas-like molecule HEF1 with CrkL following integrin and antigen receptor signaling in human B cells. Possible relevance to neoplastic lymphohematopoietic cells. Leuk. Lymphoma. 1997;28:65-72.
  • Serebriiskii I, Estojak J, Sonoda G, Testa JR, Golemis EA. Association of Krev-1/rap1a with Krit1, a novel ankyrin repeat-containing protein encoded by a gene mapping to 7q21-22. Oncogene. 1997 Aug 28;15(9):1043-9.
  • Astier A, Manie SN, Avraham H, Hirai H, Law SF, Zhang YH, Golemis EA, Fu YG, Druker BJ, Haghayeghi N, Freedman AS, Avraham S. The related adhesion focal tyrosine kinase differentially phosphorylates p130(Cas) and the Cas-like protein, p105(HEF1). J Biol Chem. 1997 Aug 8;272(32):19719-30.
  • Wang BL, Golemis EA, Kruh GD. ArgBP2, a multiple Src homology 3 domain-containing, Arg/Abl- interacting protein, is phosphorylated in v-Abl-transformed cells and localized in stress fibers and cardiocyte Z-disks. J Biol Chem. 1997 Jul 11;272(28):17542-49.
  • Sattler M, Salgia R, Shrikhande G, Verma S, Uemura P, Law SF, Golemis EA, Griffin JD. Differential signaling after beta 1 integrin ligation is mediated through binding of CRKL to p120(CBL) and p110(HEF1). J Biol Chem. 1997 May 30;272(22):14320-6.
  • Xiao GH, Shoarinejad F, Jin F, Golemis EA, Yeung RS. The tuberous sclerosis 2 gene product, tuberin, functions as a Rab5 GTPase activating protein (GAP) in modulating endocytosis. J Biol Chem. 1997 Mar 7;272(10):6097-101.
  • Manie SN, Beck AR, Astier A, Law SF, Canty T, Hirai H, Druker BJ, Avraham H, Haghayeghi N, Sattler M, Salgia R, Griffin JD, Golemis EA, Freedman AS. Involvement of p130(Cas) and p105(HEF1), a novel Cas-like docking protein, in a cytoskeleton-dependent signaling pathway initiated by ligation of integrin or antigen receptor on human B cells. J Biol Chem. 1997 Feb 14;272(7):4230-6.
  • Law SF, Estojak J, Wang BL, Mysliwiec T, Kruh G, Golemis EA. Human enhancer of filamentation 1, a novel p130(cas)-like docking protein, associates with focal adhesion kinase and induces pseudohyphal growth in Saccharomyces cerevisiae. Mol Cell Biol. 1996 Jul;16(7):3327-37.
  • Wang BL, Mysliwiec T, Krainc D, Jensen RA, Sonoda G, Testa JR, Golemis EA, Kruh GD. Identification of ArgBP1, an Arg protein tyrosine kinase binding protein that is the human homologue of a CNS-specific Xenopus gene. Oncogene. 1996 May 2;12(9):1921-9.
  • Estojak J, Brent R, Golemis EA. Correlation of 2-Hybrid Affinity Data with in-Vitro Measurements. Mol Cell Biol. 1995 Oct;15(10):5820-9.
  • Khazak V, Sadhale PP, Woychik NA, Brent R, Golemis EA. Human Rna-Polymerase-Ii Subunit Hsrpb7 Functions in Yeast and Influences Stress Survival and Cell Morphology. Mol Biol Cell. 1995 Jul;6(7):759-75.
  • Datta K, Franke TF, Chan TO, Makris A, Yang S-I, Kaplan D, Morrison DK, Golemis EA, Tsichlis PN. AH/PH domain mediated dimerization of Akt and its potential role in Akt regulation. Mol Cell Biol. 199;15:2304-10.
  • Sato T, Hanada M, Bodrug S, Irie SJ, Iwama N, Boise LH, Thompson CB, Golemis E, Fong L, Wang HG, Reed JC. Interactions among Members of the Bcl-2 Protein Family Analyzed with a Yeast 2-Hybrid System. Proc Natl Acad Sci U S A. 1994 Sep 27;91(20):9238-42.
  • Gyuris J, Golemis E, Chertkov H, Brent R. Cdi1, a human G1 and S phase protein phosphatase that associates with Cdk2. Cell. 1993;75:791-803.
  • Golemis E and Brent R. Fused moieties inhibit DNA binding by LexA derivatives. Mol Cell Biol. 1992;12:3006-14.
  • Speck N, Renjifo B, Golemis E, Frederickson TN, Hartley JW and Hopkins N. Mutation of the core or adjacent LVb elements of the Moloney murine leukemia virus enhancer alters disease specificity. Genes and Development. 1990;4(2):233-42.
  • Golemis E, Speck NA and Hopkins N. Alignment of U3 region sequences of mammalian type C viruses: Identification of highly conserved motifs and implications for enhancer design. J Virol. 1990;64(2): 534-42.
  • Golemis E, Li Y, Frederickson TN, Hartley JW and Hopkins N. Distinct segments within the enhancer region collaborate to specify the type of leukemia induced by nondefective Friend and Moloney viruses. J Virol. 1989;63(1):328-37.
  • Li Y, Golemis E, Hartley JW, and Hopkins N. Disease specificity of nondefective Friend and Moloney murine leukemia viruses is controlled by a small number of nucleotides. J Virol. 1987;61(3):693-700.

Reviews, Technical Papers

  • Bagnyukova, T., Serebriiskii, I.G., Zhou, Y., Hopper-Borge, E.A., Golemis, E.A., and Astsaturov, I. Chemotherapy and signaling: how can targeted therapies supercharge cytotoxic agents? Cancer Biol. Ther. 10(9):1-15, 2010.
  • Golemis, E.A. TACCing on new functions for the TSC2 tumor suppressor. News and Views, Cell Cycle 9:1232-1233, 2010.
  • Tikhmyanova, N., Little, J.L., and Golemis, E.A. Cas Proteins in Normal and Pathological Cell Growth Control, Cell Mol Life Sci, Epub November 25, 2009; 67:1025-1048, 2010.
  • Plotnikova OV, Pugacheva EN, and Golemis EA. 7. Primary cilia and the cell cycle. Methods in Cell Biology. 2009;94:137-60.
  • Ratushny V, Astsaturov I, Burtness BA, Golemis EA, Silverman JS. Targeting EGFR resistance networks in head and neck cancer. Cell Signalling. 2009;21:1255-68.
  • Hopper-Borge EA, Nasto RE, Ratushny V, Weiner LM, Golemis EA, Astsaturov I. Mechanisms of tumor resistance to EGFR-targeted therapies. Expert Opin Ther Targets. 2009;13:339-62.
  • Nasto R, Serebriiskii IG, Golemis EA. In silico data mining for protein network construction. Chapter 16 in "Basic Methods in Protein Purification and Analysis," Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. 327-346, 2008
  • Ratushny, V., and Golemis EA. Resolving the network of cell signaling pathways using the evolving yeast two-hybrid system. Biotechniques 44:655-662, 2008.
  • Plotnikova OV, Golemis EA, Pugacheva EN. Cell cycle-dependent ciliogenesis and cancer. Cancer Res. 2008;68:2058-61
  • Khazak V, Astsaturov I, Serebriiskii IG, Golemis EA. Selective Raf Inhibition in Cancer Therapy. Expert Opin Ther Targets. 2007;11:1587-1609.
  • O'Neill GM, Seo S, Serebriiskii IG, Lessin SR, Golemis EA. A new central scaffold for metastasis: parsing HEF1/Cas-L/NEDD9. Cancer Res. 2007;67:8975-9.
  • Singh MK, Cowell L, Seo S, O'Neill GM, Golemis EA. Molecular basis for HEF1/NEDD9/Cas-L action as a multifunctional coordinator of invasion, apoptosis and cell cycle. Cell Biochemistry and Biophysics. 2007;48:54-72.
  • Serebriiskii IG, Milech N, Golemis EA. A bacterial/yeast merged two-hybrid system: protocol for bacterial screening. Gene Function Analysis: Methods in Mol Biol. 2007;408:291-315.
  • Tikhmyanova N, Izumchenko E, Serebriiskii IG, Golemis EA. A bacterial/yeast merged two-hybrid system: protocol for yeast screening with single or parallel baits. Gene Function Analysis: Methods in Mol Biol. 2007;408:257-90.
  • Sagi O, Wolfson M, Cohen A, Izumchenko E, Golemis EA, Fraifeld VE. Shc proteins and brain functions: possible implications for neuroprotection. Int J Neuroprotection and Neuroregeneration. 2006;3:10-9.
  • Izumchenko E, Wolfson M, Golemis EA, Serebriiskii IG. Yeast hybrid approaches. Methods in Microbiol. 2007;36:103-37.
  • Pugacheva EN, Roegiers F, Golemis EA. Interdependence of cell attachment and cell cycle signaling. Curr Opin Cell Biol. 2006;18:507-15.
  • Khazak V, Kato-Stankiewicz J, Tamanoi F, Golemis EA. Yeast screens for inhibitors of Ras-Raf interaction and characterization of MCP inhibitors of Ras-Raf interaction. in Regulators and Effectors of Small GTPases. ed. C Der. Methods Enzym. 2005;407:612-29.
  • Adams PD, Golemis EA. Preface to the Second Edition. pp ix-xiii, in Protein-Protein Interactions, Second Edition: a Molecular Cloning Manual, 938 pages. Eds. Golemis EA, and Adams PD, Cold Spring Harbor Laboratory Press, New York, 2005.
  • Pugacheva EN, Golemis EA. Building a better web: progress in the concept and methodology of protein interaction studies. pp. 13-35 in Protein-Protein Interactions, Second Edition: a Molecular Cloning Manual, 938 pages. Eds. Golemis EA, and Adams PD. Cold Spring Harbor Laboratory Press, New York, 2005.
  • Khazak V, Golemis EA, Weber L. Development of a yeast two-hybrid screen for selection of human Ras-Raf protein interaction inhibitors. Chemical Genomics: Reviews and Protocols, ed. ED Zanders. Methods in Molecular Biology. 2005;310:253-71.
  • Peterson JR, Golemis EA. Autoinhibited proteins as promising drug targets, solicited 'Prospect' article for J Cell Biochem. 2004;93:68-74.
  • Serebriiskii IG, Golemis EA, Uetz P. The yeast two-hybrid system for detecting interacting proteins. Chapter 56, Methods in Molecular Biology: The Proteomics Handbook, Humana Press, 2005
  • Golemis EA, Chernoff J. Editorial: Analysis and Manipulation of Intracellular Signaling Cascades. Methods. 2004;32:347-8.
  • Serebriiskii IG, Golemis EA. Two Hybrid Protein-Protein Interactions. in Encyclopedia of Biological Chemistry, Volume 4, pp. 290-293, eds. Lennarz WJ, Lane MD, Elsevier Press, Oxford, 2004.
  • Hudes G, Menon S, Golemis EA. Protein interaction-targeted drug discovery. Ch. 15., pp 325-347, in Molecular Analysis and Genome Discovery, John Wiley & Sons, 2004.
  • Ochs MF Golemis EA. The Biology Behind Gene Expression: a basic tutorial. Methods of Microarray Analysis III, pp 9-24, Kluwer Press, 2003.
  • O'Neill GM, Catchpoole DR, Golemis EA. From correlation to causality: microarrays, cancer, and cancer treatment. Biotechniques, Suppl: pp 64-71, 2003.
  • Golemis EA, Dadke D, Tew KD. Protein interaction-targeted drug discovery: evaluating critical issues. Biotechniques. 2002;32:636-47.
  • Golemis EA, Ochs MF, Pugacheva EN. Signal transduction driving technology driving signal transduction: Factors in the design of targeted therapies. J Cell Biochem. 2001;S37:42-52.
  • Serebriiskii IG, Khazak V, Golemis EA. Redefinition of the yeast two-hybrid system in dialogue with changing priorities in biological research. BioTechniques. 2001;30:634-55.
  • Toby GG, Golemis EA. Using the yeast Interaction Trap and other two-hybrid based approaches to study protein-protein interactions. Methods. 2001;24:201-17.
  • Toby GG, Golemis EA. Targeting proteins to specific cellular compartments to optimize physiological activity. Methods in Enzymology. 2001;332:77-87.
  • Serebriiskii IG Golemis EA. Two hybrid system false positives, and approaches to their detection and elimination. Ch. 9 (pp 123-134) In Methods in Molecular Biology: Two hybrid systems, Methods and Protocols, ed. PN MacDonald. Humana Press, Totowa, NJ, 2001.
  • Serebriiskii IG, Toby GG, Finley Jr RL, Golemis EA. Genomic analysis utilizing the yeast two-hybrid system. Chapter 26 (pp 415-444) In Methods in Molecular Biology: Genomic Protocols, ed. M Starkey. Humana Press, Totowa, NJ, 2001.
  • Einarson MB, Golemis EA. Encroaching genomics: adapting large-scale science to small academic laboratories. Physiol. Genomics. 2000;2:85-92.
  • O'Neill GM, Fashena SJ, Golemis EA. Integrin signaling: a new Cas(t) of characters enters the stage. Trends in Cell Biol. 2000;10:111-9.
  • Fashena SJ, Serebriiskii IG, Golemis EA. LexA based two-hybrid systems. In Chimeric Genes and Proteins, eds. J Abelson, S Emr, J Thorner. Methods Enzymol. 2000;328:14-25.
  • Golemis E. Analysis of protein-protein interactions: An overview. Ch 18.2-18.5, in: the 3rd edition of Molecular Cloning, ed. Sambrook, J., Cold Spring Harbor Laboratory Press. 2000.
  • Golemis EA, Serebriiskii I. Review: Two hybrid and Two Component Systems. Ch. 18.6-18.16, in: the 3rd edition of Molecular Cloning, ed. Sambrook J. Cold Spring Harbor Laboratory Press. 2000.
  • Golemis EA, Serebriiskii I. Protocol. Two-hybrid approaches to analysis of protein-protein interaction. Cells: a Laboratory Manual, eds. DL Spector, R Goldman, L Leinwand. Cold Spring Harbor Laboratory Press, 1997. Updated and reprinted in:3rd edition of Molecular Cloning: a Laboratory Manual, ed. J. Sambrook, 2000.
  • Golemis E, Serebriiskii I, Gyuris J, Brent R. Interaction Trap/Two-Hybrid Systems to Identify Interacting Proteins. Unit 20.1.1-20.1.35 In: Current Protocols in Molecular Biology, ed. Ausubel FM, et al, John Wiley & Sons, NY, 1997. Updated and reprinted in: Current Protocols in| Protein Science, 1998 and Current Protocols in| Neuroscience, 1999.
  • Golemis EA, Brent R. Basic Protocol III, the Interaction Trap. p 43-71 in The Yeast Two-Hybrid System ed. S Fields and P Bartel Oxford University Press, New York. 1997.
  • Golemis EA, Serebriiskii I, Law S. Adjustment of Parameters in the yeast two-hybrid system: criteria for detecting physiologically significant protein-protein interactions. p. 11-28 in Current Innovations in Molecular Biology: Gene Cloning and Analysis, ed. B. Schaefer, Horizon Scientific Press, 1997. reprinted in: Curr. Issues Mol Biol. 1999:1(1):31-46.
  • Golemis E, Khazak V. Alternative yeast two-hybrid systems: The interaction trap and interaction mating. p197-218 in: Methods in Molecular Biology, Volume 63: Recombinant Protein Protocols, Chapter 16, ed. Tuan R, Humana Press, Clifton, 1997.
  • Golemis E, Gyuris J, Brent R. Interaction Trap/Two-Hybrid Systems to Identify Interacting Proteins. Unit 20.1.1-20.1.28 In: Current Protocols in Molecular Biology, ed. Ausubel FM, et al, John Wiley & Sons, NY, 1996.
  • Klickstein LB, Neve RL, Golemis E, and Gyuris J. Conversion of mRNA into double-stranded cDNA. In: Current Protocols in Molecular Biology, Unit 5.5.1-13, ed. Ausubel FM et al, John Wiley & Sons, New York, 1995.
  • Golemis E, Gyuris J, and Brent R. Interaction Traps: A Two-Hybrid System to Identify Interacting Proteins. Unit 13.14.1 - 13.14.17 In: Current Protocols in Molecular Biology, ed. Ausubel, FM, et al., John Wiley & Sons, NY, 1994.
  • Hopkins N, Golemis E and Speck N. Role of enhancer regions in leukemia induction by nondefective murine C-type retroviruses. In: Concepts in Viral Pathogenesis III (Oldstone, M.B.A., et al, eds.) Springer-Verlag, NY, 1989.

Editorial Projects

  • Acquisitions and Development Editor, Chapter 18, Analysis of Protein-Protein Interactions, in Molecular Cloning, 3rd Edition, ed. Sambrook, J., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY 2001.
  • E Golemis, Editor, Protein Interactions, flagship laboratory manual for a Molecular Cloning series, series editor J. Sambrook, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY 2002, 682 pp.
  • E Golemis and J Chernoff, Editors, Methods, issue entitled "Analysis and Manipulation of Intracellular Signaling Cascades." 32(4), April 2004.
  • Protein-Protein Interactions, Second Edition: a Molecular Cloning Manual. Eds. Golemis EA and Adams PD, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, 938 pages, 2005.
  • Simpson RJ, Adams PD, Golemis EA, eds. "Basic Methods in Protein Purification and Analysis," Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY., 436 pages, 2008.